Amino Acid Analysis Protocols (Methods in Molecular Biology - download pdf or read online
By Cooper C., Packer N.
Proteome structures Ltd, North Ryde, Australia. Describes various amino acid research ideas and the way every one process can be utilized to respond to particular biologic questions.
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Additional resources for Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159)
1984) Amino acid analysis by reversedphase high-performance liquid chromatography: pre-column derivatization with phenylisothiocyanate. Anal. Biochem. 136, 65–74. 5. Cohen, S. A. and Strydom, D. J. (1988) Amino acid analysis utilizing phenylisothiocyanate derivatives. Anal. Biochem. 174, 1–16. 6. Cohen, S. A. and Michaud, D. P. (1993) Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high performance liquid chromatography.
It is wise to monitor the recovery of the protein by absorbance of the column eluent at 280 nm. Collect sample until all of the material is eluted. 8. When samples containing a high concentration of protein or peptide are available in reasonable volume, there is no need to purify the sample further, simply dilute the sample with HPLC-grade water to a salt concentration that will not interfere with the analysis. 9. Acetonitrile (ACN) concentrations greater than 15% interfere with sample binding to PVDF.
A. and De Antonis, K. M. (1994) Applications of amino acid analysis derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Analysis of feed grains, intravenous solutions and glycoproteins. J. Chromatog. 661, 25–34. 11. Van Wandelen, C. and Cohen, S. A. (1997) Using quaternary high-performance liquid chromatography eluent systems for separating 6-aminoquinolyl-N- Precolumn Derivatization with AQC 47 hydroxysuccinimidyl carbamate-derivatized amino acid mixtures. J. Chromatog. 763, 11–22.
Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159) by Cooper C., Packer N.