New PDF release: Biological Dosimetry: Cytometric Approaches to Mammalian
By David C. Lloyd (auth.), Priv.-Doz. Dr. Dr. Wolfgang G. Eisert, Dr. Mortimer L. Mendelsohn (eds.)
In October 1982, a small overseas symposium was once held on the Gesellschaft fUr Strahlen- und Umweltforschung mbH (GSF) in Munich as a satellite tv for pc assembly of the IX overseas convention on Analytical Cytology. The symposium focussed on cytometric ways to organic dosimetry, and was once, to the simplest of our wisdom, the 1st assembly in this topic ever held. there has been powerful encouragement from the seventy five attendees and from others to post a complaints of the symposium. accordingly this ebook, containing 30 of the 36 shows, has been assembled. Dosimetry, the actual and systematic selection of doses, often refers to grams of substance administered or rads of ionization or a few such degree of publicity of a sufferer, a sufferer or an experimental approach. The time period may also be used to explain the amount of an final, energetic agent as dropped at the right goal fabric inside of a organic procedure. hence, for mutagens, you'll be able to converse of DNA dosimetry, that means the variety of adducts produced within the DNA of aim cells reminiscent of bone-mar row stem cells or spermatogonia.
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Additional info for Biological Dosimetry: Cytometric Approaches to Mammalian Systems
Bender MA, Gooch PC (1962) Types and rates of X-ray-induced chromosome aberrations in human blood irradiated in vitro. ) 48:522-532 8. Bender MA (1969) Human radiation Cytogenetics. Adv. Radiat Bioi 3:215-275 9. Countryman P, Heddle J (1976) The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutation Res 41:321-331 24 M. Bauchinger 10. Crossen PE, Morgan WF (1977) Analysis of human lymphocyte cell cycle time in culture measured by sister chromatid differential staining.
3 Gy), the method is not sensitive enough to detect a significant increase in the incidence of micronuclei as compared to controls. 4 o 48 h 72 h o 03 84 h 0; u 0; a. 0 Dose [Gy] Fig. 9. Linear dose-effect curves for micronuclei induced by 220 keY X-rays in human lymphocytes However, a variant of the method might be useful in detecting potential radiosensitive radiation workers. In this approach, the worker's cells are exposed to radiation at high levels of dose in vitro and the yield of micronuclei is checked for excessively high responses.
0, 2D and 4D Gy of X-irradiation. In this experiment, the cells were growing exponentially in monolayer culture at the time of irradiation. The cells were returned to culture for 20 h after irradiation and then treated for 4 h with colcemid. , 1980) and stained with the DNA specific dye propidium iodide. At least 4000 profiles were recorded for chromosomes from cells taken at each dose point using the Livermore slit-scan flow cytometer. During these analyses, the slit-scan flow cytometer was adjusted so that profiles were recorded only for chromosomes larger than the number two chromosome.
Biological Dosimetry: Cytometric Approaches to Mammalian Systems by David C. Lloyd (auth.), Priv.-Doz. Dr. Dr. Wolfgang G. Eisert, Dr. Mortimer L. Mendelsohn (eds.)